Generally for stage 1 you shouldn't need any modifications. Stage 1 is normally just an ecu flash.
For Stage 2 you generally require bolt ons- DP, IC, intake. Fueling can be needed but generally is 2+.
E30 is just adding 15% more ethanol to 90% of pump blends out there. Almost all pump gas is a 15% ethanol blend anymore. You can run e30 on stock hardware for knock if you wanted. A tune would just give you more (relative, don't expect huge gains on e30) timing, boost, or both from the increased octane.
R has the largest DI injectors of the 3 Golf's, so they can get away with more on stock fueling. If going larger than IS38, I'd personally recommend LPFP and MPI. You can do both for under $1800 total. OEM+ MPI is 600-800 and includes manifold and everything else needed to be plug and play. Rs3 Lpfp is $1075.
If not going for more power, a HPFP e85 stage 2 tune would work just fine IIRC.